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Materials for delivery of oligonucleotides need to be simple to produce yet effective in vivo to be considered for clinical applications. Formulations of biomaterials based on combinations of existing demonstrated polymeric gene carriers with targeted derivatives are potential candidates for rapid translation but have not been fully explored for siRNA applications. Here we investigated formulations based on derivatised PEI for delivery of siRNA to gastrointestinal cancer cells.
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Several experiments were then conducted to establish a just meaningful difference (JMD) for SNR. SNR changes that could induce intervention seeking behaviour for an individual were measured with subjective scaling and report, using the same stimuli as the SNR JND experiment as pre and post benefit examples. The results across different rating and willingness to change tasks showed that the mean ratings increased near linearly with a change in SNR, but a change of at least 6 dB was necessary to reliably motivate participants to seek intervention.