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Last April, I attended a conference sponsored by the American Composers Forum, of which I a member. It was titled On and covered the topic of composing for video games. On the first evening of the event, I had the privilege of hearing the Alpine Quartet perform some selections from the soundtrack to Red Orchestra 2: Rising Storm, composed by Lennie Moore.
The effect of several pertinent, thermostabilizing point mutations was assessed by reverting them to the native sequence. Whereas the response to ligands remains largely unchanged, binding of the G protein mimetic nanobody NB80 and G protein activation are only observed when two conserved tyrosines (Y227 and Y343) are restored. Binding of NB80 leads to very strong spectral changes throughout the receptor, including the extracellular ligand entrance pocket.
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